Spectroscopic Studies on the Interaction of Nano-TiO2 with Lysozyme

Authors

  • B. Shareghi Department of Biology, University of Shahrekord, P.O. Box: 115, Shahrekord, I. R. of Iran
  • M. Salavati-Niasari Institute of Nano Science and Nano Technology, University of Kashan, Kashan, P.O.BOX.87317-51167,I. R. of Iran
  • R. Amooaghaei Department of Biology, University of Shahrekord, P.O. Box: 115, Shahrekord, I. R. of Iran
  • S. Farhadian Department of Biology, University of Shahrekord, P.O. Box: 115, Shahrekord, I. R. of Iran
Abstract:

In the present study, the interaction between nano-TiO2 and lysozyme was investigated by the method of UV-Vis detection and fluorescence spectroscopic techniques. The thermal denaturation of lysozyme has been investigated in the presence and absence of nano-TiO2 over the temperature range (293-373) K in different buffer and pH, using temperature scanning spectroscopy. The presence of nano-TiO2 caused the destabilization of lysozyme resulting in a decrease in the temperature of unfolding with an increase in nano-TiO2 concentration.

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Journal title

volume 1  issue 2

pages  95- 103

publication date 2011-04-01

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